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The aqueduct of midbrain, is within the mesencephalon, contains cerebrospinal fluid, and connects the third ventricle in the diencephalon to the fourth ventricle within the region of the mesencephalon and metencephalon, located dorsal to the pons and ventral to the cerebellum.
The aqueduct functions to connect the third and fourth ventricles and to ensure the flow of cerebrospinal fluid through these areas.